Barcelona captain, Lionel Messi, has been ruled out of their game against Real Betis on Sunday, after suffering a setback in his return from a calf injury, ESPN reports.
Messi originally sustained the knock earlier this month and missed Barca's tour of the United States.
He also say out their 1-0 defeat to Athletic Bilbao in the league last Friday.
Messi returned to full training earlier this week and was expected to be in the squad for Betis' visit this weekend, but felt some discomfort when training on Friday.
Barca then took the decision for the Argentina forward to train alone on Saturday.
Manager Ernesto Valverde has said he will not take unnecessary risks by rushing Messi back, despite the fact that Barca are already missing Luis Suarez and Ousmane Dembele due to injuries.